![]() ![]() The contents of tar and nicotine for each brand were determined by the method developed by International Standardization Organisation (ISO). After completion of smoking, the Cambridge filters used were removed and disintegrated completely in methanol. Appropriate length of cigarette was smoked leaving butt length as indicated in Table I. Puff and puff duration was so adjusted that a complete cycle lasted 60 seconds, giving a puff frequency of one per minute. Mainstream smoke analysis of 47 brands of commercial cigarettes was done using 8 channel Cigarettes Smoking Machine (Model SM-302 supplied by M/S Filtrona Instruments and Automation Ltd., England) provided by WHO.
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